AccuBlotTM anti-Rabbit IgG-HRP
Croyez AccuBlotTM anti-Rabbit IgG-HRP is conjugated with high-activity HRP.
In co-immunoprecipitation (co-IP) assay, denatured IgG from IP samples may
shows interference result in immunoblotting at ~55 kDa (heavy chain) and ~25
kDa (light chain) by using traditional secondary antibodies. The products of
AccuBlotTM series are suitable in native IgG detection.
In co-immunoprecipitation (co-IP) assay, denatured IgG from IP samples may
shows interference result in immunoblotting at ~55 kDa (heavy chain) and ~25
kDa (light chain) by using traditional secondary antibodies. The products of
AccuBlotTM series are suitable in native IgG detection.
Product type:
Monoclonal
Immunogen:
Purified rabbit IgG
Host:
Mouse
Reactivity:
Native rabbit IgG
Conjugation:
HRP (RZ>3)
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.
Storage:
Protect from light.
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation.
Application:
ELISA, WB
Manual:
1:1000-1:3000 for WB
1:2500-1:10000 for ELISA
Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice
Monoclonal
Immunogen:
Purified rabbit IgG
Host:
Mouse
Reactivity:
Native rabbit IgG
Conjugation:
HRP (RZ>3)
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.
Storage:
Protect from light.
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation.
Application:
ELISA, WB
Manual:
1:1000-1:3000 for WB
1:2500-1:10000 for ELISA
Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice
1.
Lai YC, Cheng YW, Chao CH, et al. Antigenic Cross-Reactivity Between SARS-CoV-2 S1-RBD and Its Receptor ACE2. Frontiers in Immunology. 2022 ;13:868724.
Lai YC, Cheng YW, Chao CH, et al. Antigenic Cross-Reactivity Between SARS-CoV-2 S1-RBD and Its Receptor ACE2. Frontiers in Immunology. 2022 ;13:868724.